OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
The lck protooncogene is a member of the src-related gene family of non-receptor tyrosine kinases. It is uniquely expressed only in T-lymphocytes and NK-cells. The p56lck kinase (LCK) is a 56 KD polypeptide that is associated with a cytoplasmic face of the plasma membrane. It functions as a critical signalling model in IL-2 signal transduction leading to T-lymphocyte activation. Specifically, it has been shown that a stable complex exists between IL-2 receptors and p56lck and that treatment of T-lymphocytes with IL-2 promotes p56lck kinase activity and rapid phosphorylation of cellular substrates. In addition, p56lck has been found to function as a signal transducer for antigen activated CD4 and CG8 T-cell receptors and plays a role in thymic maturation of certain T-cells. The CD45 antigen, a receptor/protein tyrosine phosphatase of T-cells, also interacts with p56lck.
Family:
Kinase
Sub Family:
TK
Class:
Protein Tyrosine
Protein Name:
Lck
Uniprot Number:
P06239
Protein Aliases:
Leukocyte C-terminal Src kinase|LSK|Lymphocyte cell-specific protein-tyrosine kinase|Protein YT16|Proto-oncogene Lck|T cell-specific protein-tyrosine kinase|p56-LCK
Gene Name:
LCK
Gene ID:
3932
Organism:
Human
Construct Details:
Full length recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Procedure Summary:
Lck (h) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 250 µM KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [gamma-33P]-ATP (specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of phosphoric acid to a concentration of 0.5%. An aliquot of the reaction is then spotted onto a filter and washed four times for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting.
Substrate:
250 µM KVEKIGEGTYGVVYK (Cdc2 peptide)
Tracer:
33P
ATP Concentration:
[ATP]= 90µM (Km= 95µM )
Incubation:
40 min at Room temperature
Control Inhibitor:
Staurosporine
Test Sample Requirements:
Please provide compounds dissolved in 100% DMSO (PBS or HBSS prohibited).
Stock solution should be ≥50× the final assay concentration (a 10 mM stock is also acceptable).
Required volume:
– <60 kinase assays: 120 µL of 50× stock
– ≥60 assays: 420 µL of 50× stock
If you supply 10 mM stocks or powder (pre-weighed), we will apply the appropriate conversion.
For batches of ~50 compounds supplied as solids, the TAT will be extended.
Minimum Order Quantity:
2
Turnaround Time
Standard:
6 Business Days
Service Scheduling Note:
Assay is run on a weekly schedule beginning on Friday. Compounds and purchase order must be received on or before 13:00 CET Thursday for the order to be included on the service run that begins on Friday of the same week.
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Adverse / Beneficial Action:
Inhibition of LCK may result in immune suppression and apoptosis.
Additional Information
Brand:
LeadHunter
Testing Location:
KP - France - Celle L'Evescault
RELATED SOLUTIONS
Lck Human TK Kinase Enzymatic Radiometric [10 uM ATP] KinaseProfiler LeadHunter Assay - FR
Item: 14-442KP10
Lck (activated) Human TK Kinase Enzymatic Radiometric [Km ATP] KinaseProfiler LeadHunter Assay - FR
Item: 14-842KP
Lck (activated) Human TK Kinase Enzymatic Radiometric [10 uM ATP] KinaseProfiler LeadHunter Assay - FR
Item: 14-842KP10
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
