15-LOX-2 Human Lipoxygenase Enzymatic LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
15-Lipoxygenase initiates the metabolic pathway leading to the formation of lipoxins by converting arachidonic acid to 15-hydroxyperoxy-eicosatetraenoic acid (15-HPETE). Lipoxins may share some of the bioactivity associated with leukotrienes.
Family:
Lipid Metabolism
Sub Family:
Eicosanoid Turnover
Class:
Lipoxygenase
Protein Name:
15-LOX-2
Uniprot Number:
O15296
Protein Aliases:
Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-LOX-2, Arachidonate 15-lipoxygenase type II
Gene Name:
ALOX15B
Gene ID:
U78294
Accession Number:
NM_001039130
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Fluorimetry
Measured Response:
Fluorescence
Testing Information
Substrate:
Arachidonic Acid (10 µM)
Incubation:
90 min at 30°C
Control Inhibitor:
2-TEDC
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
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