B2 Human Bradykinin GPCR Cell Based Antagonist Calcium Flux LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Bradykinin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Bradykinin receptors are found in most peripheral tissues and within the central nervous system. Bradykinin B2 receptor agonism may promote algesia, cough, bronchoconstriction, inflammation and vasodilation. Bradykinin B2 receptor antagonism may find therapeutic use in the treatment of chronic pain and inflammation.
Family:
GPCR
Sub Family:
Bradykinin
Class:
Class A
Protein Name:
B2
Uniprot Number:
P30411
Protein Aliases:
BK-2 receptor
Gene Name:
BDKRB2
Gene ID:
624
Gene Aliases:
BK-2|BKR2
Accession Number:
NM_000623
Organism:
Human
Construct Details:
Recombinant
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
Fluorimetry
Measured Response:
Fluorescence
Testing Information
Incubation:
RT
Control Inhibitor:
HOE140
Control Activator:
bradykinin (0.03 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 70 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Adverse / Beneficial Action:
Bradykinin B2 receptor agonism may facilitate algesia, cough, bronchoconstriction, inflammation and vasodilation. Bradykinin B2 receptor antagonism may find therapeutic use in the treatment of chronic pain and inflammation.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
B2 Human Bradykinin GPCR Binding Agonist Radioligand LeadHunter Assay - FR
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B2 Human Bradykinin GPCR Cell Based Agonist Calcium Flux LeadHunter Assay - FR
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B2 Human Bradykinin GPCR Cell Based Agonist & Antagonist Calcium Flux LeadHunter Assay - FR
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