Biological Information

Background Information:

Bombesin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Bombesin receptors are widely distributed in the central nervous system and peripheral tissues.

Family:

GPCR

Sub Family:

Bombesin

Class:

Class A

Protein Name:

BB3

Uniprot Number:

P32247

Gene Name:

BRS3

Gene ID:

680

Gene Aliases:

BB3|BB3R

Accession Number:

NM_001727

Organism:

Human

Construct Details:

Recombinant

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Binding

Detection Method:

Radiometric

Measured Response:

Scintillation

Testing Information

Ligand:

[125I]Bn(6-14)

Ligand Kd (nM):

0.16

Ligand Concentration:

0.1 nM

Non Specific:

1 µM Bn (6-14)

Incubation:

60 min at RT

Control Inhibitor:

Bn(6-14)

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

16130995

Name

bombesin

Measurement

9500nM - IC50

PubChem ID

Name

Bn(6-14)

Measurement

0.78nM - IC50

PubChem ID

329799973

Name

GRPh

Measurement

- IC50

PubChem ID

6324606

Name

Neuromedin B

Measurement

560nM - IC50

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Adverse / Beneficial Action:

Bombesin BB3 receptor agonism may suppress food intake and promote pulmonary tumors. Receptor antagonism may cause hyperphagia, obesity and hypertension.

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

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