Biological Information
Background Information:
Bombesin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Bombesin receptors are widely distributed in the central nervous system and peripheral tissues.
Family:
GPCR
Sub Family:
Bombesin
Class:
Class A
Protein Name:
BB3
Uniprot Number:
P32247
Gene Name:
BRS3
Gene ID:
680
Gene Aliases:
BB3|BB3R
Accession Number:
NM_001727
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[125I]Bn(6-14)
Ligand Kd (nM):
0.16
Ligand Concentration:
0.1 nM
Non Specific:
1 µM Bn (6-14)
Incubation:
60 min at RT
Control Inhibitor:
Bn(6-14)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Adverse / Beneficial Action:
Bombesin BB3 receptor agonism may suppress food intake and promote pulmonary tumors. Receptor antagonism may cause hyperphagia, obesity and hypertension.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS