OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Pam3CSK4 (Diacylated Lipoprotein) is used to specifically activate TLR1/2 and stimulates secretion of IL-6; inhibition by client compound is reported as pg/mL for the strength of the response. Reference compound dexamethasone is used for comparison purposes. Non-treated cells serve as background control, while Pam3CSK4 (Diacylated Lipoprotein) stimulated PBMCs in the presence of vehicle serve as a positive control. Human PBMCs are from a single donor in this assay.
Family:
Cell Surface (Other)
Sub Family:
TLR
Class:
PRR
Organism:
Human
Construct Details:
Endogenous
Cell Type:
PBMC
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
Luminex
Measured Response:
Fluorescence
Testing Information
Control Inhibitor:
Dexamethasone
Control Activator:
Pam3CSK4
Test Sample Requirements:
100 µl of 1000X stock based on the top concentration to be tested -OR- Enough compound to make the described solution. Minimum 1mg.
Minimum Order Quantity:
3
Turnaround Time
Standard:
20 Business Days
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
Panlabs
Testing Location:
USA - St Charles, MO
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