OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
T cell receptor activation on Jurkat cells is induced by anti-CD3 antibody to produce substantial amounts of IL-2. Test compound is evaluated for inhibitory ability and compared to the reference control dexamethasone. Non-treated cells serve as background control, while anti-CD3 stimulated Jurkat cells in the presence of vehicle serve as positive control.
Organism:
Human
Construct Details:
Endogenous
Cell Type:
Jurkat
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
Luminex
Measured Response:
Fluorescence
Testing Information
Incubation:
24 hrs at
Control Inhibitor:
Dexamethasone
Control Activator:
Anti-CD3 antibody
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Turnaround Time
Standard:
20 Business Days
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
Panlabs
Testing Location:
USA - St Charles, MO
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