M2 Human Acetylcholine (Muscarinic) GPCR Cell Based Agonist & Antagonist cAMP LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Muscarinic receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Muscarinic receptors are widely distributed in the central and peripheral nervous system as well as various tissues.
No. of Assays on Panel:
2
Assays on Panel:
Family
GPCR
Name
M2 Human Acetylcholine (Muscarinic) GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item
1658
Protein
M2
Organism
Human
Assay Sub Type
Cell Based
Family
GPCR
Name
M2 Human Acetylcholine (Muscarinic) GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
Item
1659
Protein
M2
Organism
Human
Assay Sub Type
Cell Based
Family:
GPCR
Sub Family:
Acetylcholine (Muscarinic)
Class:
Class A
Protein Name:
M2
Uniprot Number:
P08172
Protein Aliases:
acetylcholine receptor, muscarinic 2
Gene Name:
CHRM2
Gene ID:
1129
Organism:
Human
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Agonist & Antagonist
Detection Method:
HTRF
Testing Information
Incubation:
10 min at 37°C
Control Activator:
acetylcholine (300 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Clinical Relevance
Therapeutic Area:
Cardiovascular
Adverse / Beneficial Action:
Muscarinic M2 subtype receptor agonism may cause bradycardia and hypotension. Receptor antagonism may cause airways hyper-reactivity and tachycardia.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
M2 Human Acetylcholine (Muscarinic) GPCR Binding Antagonist Radioligand LeadHunter Assay - FR
Item: 93
M2 Human Acetylcholine (Muscarinic) GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item: 1658
M2 Human Acetylcholine (Muscarinic) GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
Item: 1659
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