OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
The absorption of orally administered drugs requires the movement of the drug across the intestinal epithelial barrier. Intestinal permeability is a critical characteristic that determines the rate and extent of in vivo absorption and is correlated with the bioavailability of a drug candidate. The Caco-2 cell line is a human colon adeno-carcinoma cell line that differentiates in culture and resembles the epithelial lining of the human small intestine. It has been widely used as an in vitro model for drug transport and permeability screening of discovery compounds.
Organism:
Human
Construct Details:
Endogenous
Cell Type:
Caco-2
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Detection Method:
HPLC-MS/MS
Measured Response:
Peak Area Response
Testing Information
Substrate:
Test compound
Incubation:
0 and 60 min at 37°C
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
10 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
RELATED SOLUTIONS
P-gp substrate assessment (Caco-2) - US
Item: g206
Bidirectional permeability (Caco-2; pH 7.4/7.4) - US
Item: g236
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