Acetyl-coenzyme A carboxylase 2 Human Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Acetyl-CoA carboxylase 2 (ACC2), also known as ACC-beta, is a biotin-dependent enzyme that catalyzes the conversion of acetyl-CoA to malonyl-CoA, a key step in fatty acid metabolism. As one of two ACC isoforms, ACC2 is primarily expressed in oxidative tissues such as the heart, skeletal muscle, and liver, where it localizes to mitochondria. It regulates fatty acid β-oxidation by producing malonyl-CoA, which inhibits carnitine palmitoyltransferase I (CPT1), the rate-limiting enzyme for mitochondrial fatty acid uptake. This inhibition reduces fatty acid oxidation and helps balance lipid storage and utilization. ACC2 is inhibited by AMP-activated protein kinase (AMPK) during energy stress. Dysregulation of ACC2 contributes to metabolic disorders, including obesity, type 2 diabetes, fatty liver, and cardiovascular disease. Its pivotal role in lipid balance and energy regulation makes ACC2 a compelling therapeutic target for metabolic disease intervention.
Family:
Lipid Metabolism
Class:
Lipid metabolism
Accession Number:
NM_001093
Organism:
Human
Construct Details:
Full length, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Luminescence
Measured Response:
Luminescence
Testing Information
Procedure Summary:
Human recombinant ACC2 enzyme expressed in Sf9 cells is used. Test compound and/or vehicle is preincubated with 0.50 μg/ml active enzyme in modified HEPES buffer pH 7.4 for 15 minutes at 25oC. The reaction is initiated by addition of 20 µM Acetyl-CoA for another 30 minutes incubation period. Determination of the amount of ADP formed by ADP-Glo™ Kinase Assay Kit is read luminescence on a spectrophotometer. Compounds are screened at 10 μM.
Substrate:
20 µM Acetyl-CoA
Incubation:
30 min at 25°C
Control Inhibitor:
Firsocostat
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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