BLT1 (LTB4) Human Leukotriene GPCR Cell Based Agonist Calcium Flux (in Arrestin Cell Line) LeadHunter Assay - US

Background Information:

Leukotriene (LT) receptors most sensitive to the endogenous ligand BLT4 are named BLT receptors, whereas those preferentially activated by the CysLTs are named LTC4, D4 and E4. LT receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. LTB4 receptors are found in leukocytes, spleen and thymus and also reported to be present in peritoneal macrophages and eosinophils. LTB4 receptor agonism promotes neutrophil chemotaxis and secretion contributing to inflammation, but may also affect immunomodulation, etc. BLT receptor antagonism may be useful in the treatment of pulmonary inflammation (COPD, asthma) and ulcerative colitis.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization.

Family:

GPCR

Sub Family:

Leukotriene

Class:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

BLT1 (LTB4)

Uniprot Number:

Q15722

Protein Aliases:

LTB4-R1|Chemoattractant receptor-like 1|G-protein coupled receptor 16|P2Y purinoceptor 7|P2Y7

Gene Name:

LTB4R

Gene ID:

1241

Gene Aliases:

BLT|BLT1|BLTR|CMKRL1|GPR16|P2RY7

Accession Number:

AY322535

Organism:

Human

Cell Type:

CHO-K1