BTK Human TK Kinase Cellular TE NanoBRET LeadHunter Assay - FR

Background Information:

The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays are based on the Bioluminescence Resonance Energy Transfer (BRET) system, an energy transfer technique designed to measure molecular proximity in living cells. The assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tracer, reversibly bound to a NanoLuc® luciferase-kinase fusion expressed in cells.

Family:

Kinase

Sub Family:

TK

Class:

Protein Tyrosine

Protein Name:

BTK

Uniprot Number:

Q06187

Protein Aliases:

Agammaglobulinemia tyrosine kinase|ATK|B-cell progenitor kinase|BPK|Bruton tyrosine kinase

Gene Name:

BTK

Gene ID:

695

Gene Aliases:

AT| ATK| BPK| XLA| IMD1| AGMX1| IGHD3| PSCTK1

Accession Number:

NM_000061

Organism:

Human

Construct Details:

NanoLuc® luciferase fused to the C-terminus of the Wild Type Full-length kinase

Cell Type:

293H