CRF1 Mouse Corticotropin-Releasing Factor GPCR Cell Based Agonist cAMP (TR-FRET in Arrestin Cell Line) LeadHunter Assay - US

Background Information:

Corticotropin-releasing factor (CRF, interchangeable with corticotropin-releasing hormone, CRH) receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. Two major subtypes of the CRF receptor have been termed the CRF1 and CRF2 receptors. The CRF1 receptor is abundantly expressed in the central nervous system with major expression sites in cortex, cerebellum, hippocampus, amygdala, olfactory bulb, and pituitary. CRF1 receptor antagonism may block stress-related responses and be useful in the treatment of anxiety, depression and gastrointestinal distress.
PathHunter β-Arrestin GPCR cell lines measure intracellular cAMP using TR-FRET technology. The PathHunter cell line used in this assay are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin.

Family:

GPCR

Sub Family:

Corticotropin-Releasing Factor

Class:

Class B

Coupling:

Gs

Protein Name:

CRF1

Uniprot Number:

P35347

Protein Aliases:

CRF-R1|CRFR-1|Corticotropin-releasing hormone receptor 1|CRH-R-1|CRH-R1

Gene Name:

CRHR1

Gene ID:

12921

Gene Aliases:

Crhr

Accession Number:

NM_007762

Organism:

Mouse

Cell Type:

CHO-K1