CX3CR1 Mouse Chemokine GPCR Cell Based Antagonist Arrestin LeadHunter Assay - US

Background Information:

Chemokine receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Chemokine receptors are primarily distributed on cells involved in immune and inflammatory function. CX3CR1 isoforms were found to serve as a coreceptors for HIV-1 and HIV-2 envelope fusion and virus infection. CX3CR1 mediates leukocyte migration and adhesion.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Chemokine

Class:

Class A

Coupling:

Gi/Go

Protein Name:

CX3CR1

Uniprot Number:

Q9Z0D9

Protein Aliases:

CX3CR1|Beta chemokine receptor-like 1|CMK-BRL-1|CMK-BRL1|Fractalkine receptor|G-protein coupled receptor 13|V28

Gene Name:

CX3CR1

Gene ID:

13051

Gene Aliases:

CMKDR1|V28|CCRL1|CMKBRL1|GPR13

Accession Number:

NM_009987.3

Organism:

Mouse

Cell Type:

CHO-K1