OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Drug-induced toxicity is one of the major causes of failure during drug development and the major reason for removal of approved drugs from market. Animal toxicity testing is an essential part of drug safety testing, but it is impractical in the early stages of drug discovery. Classic in vitro toxicity assays are not very predictive of in vivo toxicity because they measure non-specific and late occurring cytotoxic events. To fill this void in in vitro toxicity testing, Eurofins offers an image-based high content assay which measures sensitive cellular parameters such as mitochondrial membrane potential and intracellular free calcium, together with more classic parameters such as membrane permeability and cell proliferation, in live HepG2 cells.
No. of Assays on Panel:
5
Assays on Panel:
Family
Name
Nuclear size - US
Item
1562
Protein
Organism
Human
Assay Sub Type
Cell Based
Family
Name
Mitochondrial membrane potential - US
Item
1563
Protein
Organism
Human
Assay Sub Type
Cell Based
Family
Name
Intracellular free calcium - US
Item
1564
Protein
Organism
Human
Assay Sub Type
Cell Based
Family
Name
Membrane permeability - US
Item
1565
Protein
Organism
Human
Assay Sub Type
Cell Based
Family
Name
Cell number (cytotox) - US
Item
1566
Protein
Organism
Human
Assay Sub Type
Cell Based
Organism:
Human
Construct Details:
Endogenous
Cell Type:
HepG2
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Detection Method:
Imaging / HCA
Platform:
MD ImageXpress® Micro
Measured Response:
Fluorescence
Testing Information
Incubation:
72 hr at 37C
Test Concentration / Dose:
Suggested to perform dose response study (8 concentrations; 3 replicates per concentration)
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
15 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
