OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Eurofins Panlabs provides an effective in vitro cell-based screening model to assess hepatotoxicity potential of drugs with multiplexed technologies examining human primary hepatocytes (HPH) and measuring morphological and biochemical parameters at the single cell level. This assay provides the access to automated, robust, state-of-the-art technology that maximizes data per assay by measuring critical markers of liver toxicity: cell viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP). Compound testing is conducted in pre-validated human primary hepatocytes (HPH).
Organism:
Human
Construct Details:
Endogenous
Cell Type:
Hepatocytes
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Detection Method:
Imaging / HCA
Platform:
MD ImageXpress® Micro
Measured Response:
Fluorescence
Testing Information
Incubation:
72 hr at 37C
Test Concentration / Dose:
Suggested to perform dose response study (10 concentrations; 2 replicates per concentration)
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
20 Business Days
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
Panlabs
Testing Location:
USA - St Charles, MO
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
