OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Drug-induced liver injury (DILI) is one of the major reasons that drugs are withdrawn from market. Human primary hepatocytes (HPH) are currently considered as the gold standard in vitro model system to study drug induced hepatotoxicity. Lactate dehydrogenase (LDH) released into the culture medium upon plasma membrane damage is considered as one of the hallmarks of cytotoxicity. Eurofins performs the cytotoxicity assessment using cryopreserved platable human primary hepatocytes from single donor. LDH-Glo (Promega) is used as a cytotoxicity assessment.
No. of Assays on Panel:
2
Assays on Panel:
Family
Name
Cell Viability (Human Primary Hepatocytes, 24-hour, CellTiter-Glo) - US
Item
5137
Protein
Organism
Human
Assay Sub Type
Cell Based
Family
Name
Cytotoxicity (Human Primary Hepatocytes 24-hour LDH-Glo) - US
Item
5879
Protein
Organism
Human
Assay Sub Type
Cell Based
Organism:
Human
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Testing Information
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
10 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
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