delta (DOP) Human Opioid GPCR Cell Based Antagonist Arrestin LeadHunter Assay - US

Background Information:

Opiates exert their effects in the central and peripheral nervous systems through interaction with m, k and d, three major types of opioid receptors, which are structural homologues and belong to the G protein-coupled receptor (GPCR) family. G-protein cascades activated by these three receptors can reduce adenylyl cyclase activity, alter inositol trisphosphate turnover, activate G-protein-linked, inward potassium channels, and close calcium channels. These receptors mediate the actions of opiate drugs and endogenous opioid neuropeptides in producing euphoria, modulating pain perception, and altering other important functions in the central and peripheral nervous systems. Opioid analgesics provide symptomatic relief of pain, cough and diarrhea.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Opioid

Class:

Class A

Coupling:

Gi/Go

Protein Name:

delta (DOP)

Uniprot Number:

P41143

Protein Aliases:

DOR-1

Gene Name:

OPRD1

Gene ID:

4985

Gene Aliases:

OPRD

Accession Number:

NM_000911

Organism:

Human

Cell Type:

CHO-K1