OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Family:
Kinase
Sub Family:
RTK
Class:
Protein Tyrosine
Protein Name:
EPHA6
Uniprot Number:
Q9UF33
Protein Aliases:
EPH homology kinase 2 (EHK-2);EPH-like kinase 12 (EK12)
Gene Name:
EPHA6
Gene ID:
285220
Gene Aliases:
EHK2, HEK12
Accession Number:
NM_001080448.3
Organism:
Human
Construct Details:
683-end recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Procedure Summary:
Epha6 (h) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.5 mM MnCl2, 5 mM DTT, 2.5 mM Na-β-glycerophosphate, 0.1 mg/mL poly(Glu, Tyr) 4:1, 10 mM Magnesium acetate and [γ-33P]-ATP (specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of phosphoric acid to a concentration of 0.5%. An aliquot of the reaction is then spotted onto a filter and washed four times for 4 minutes in 0.425% phosphoric acid and once in ethanol prior to drying and scintillation counting.
Substrate:
0.1 mg/mL poly(Glu, Tyr) 4:1
Tracer:
33P
ATP Concentration:
[ATP]= 15µM (Km= 13µM )
Incubation:
40 min at Room temperature
Control Inhibitor:
Staurosporine
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 50X stock based on the top concentration to be tested -OR- 100 μl of 10 mM stock -OR- 1.5 mg (pre-weighed). 2) Dose Response: 80 μl of 50X stock based on the top concentration to be tested -OR- 100 μl of 10 mM stock -OR- 1.5 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
6 Business Days
Service Scheduling Note:
Assay is run on a weekly schedule beginning on Friday. Compounds and purchase order must be received on or before 13:00 CET Thursday for the order to be included on the service run that begins on Friday of the same week.
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
KinaseProfiler
Testing Location:
KP - France - Celle L'Evescault
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