GHSR Human Ghrelin GPCR Cell Based Antagonist Calcium Flux (in Arrestin Cell Line) LeadHunter Assay - US

Background Information:

The receptor for GHS (GHS-R) is a member of the superfamily of G-protein-coupled receptors. Ghrelin, an endogenous 28 amino acid present in the stomach, acts as a specific endogenous ligand for the GHS-R. Ghrelin has demonstrated potent and reproducible GH-releasing activity as well as significant prolactin-, ACTH- and cortisol-releasing activity. Its major physiological function may relate to energy homeostasis.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization.

Family:

GPCR

Sub Family:

Ghrelin

Class:

Class A

Coupling:

Gq

Protein Name:

GHSR

Uniprot Number:

Q92847

Protein Aliases:

GH-releasing peptide receptor|GHRP|Ghrelin receptor

Gene Name:

GHSR

Gene ID:

2693

Accession Number:

NM_198407

Organism:

Human

Cell Type:

U2OS