GLP-1 Human Glucagon GPCR Cell Based PAM Arrestin1 LeadHunter Assay - US

Background Information:

Glucagon-like peptide-1 (GLP-1) receptors belong to the superfamily of G protein-coupled seven transmembrane proteins.  G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells.  Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species.  GLP-1 receptors are widely expressed in brain and peripheral tissues.  GLP-1 receptor agonism may stimulate insulin and somatostatin release, inhibit gastric emptying and upper gastrointestinal transit.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Glucagon

Class:

Class B

Coupling:

Gs

Protein Name:

GLP-1R

Uniprot Number:

P43220

Protein Aliases:

GLP-1 receptor|GLP-1-R

Gene Name:

GLP1R

Gene ID:

2740

Gene Aliases:

Accession Number:

NM_002062.3

Organism:

Human

Cell Type:

CHO-K1