Biological Information

Background Information:

cAMP Hunter™ Gi cell lines overexpress naturally Gi coupled, wild type GPCRs and are designed to detect inhibition of intracellular cAMP production in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay Detection Kit.

Family:

GPCR

Sub Family:

Hydroxycarboxylic Acid

Class:

Class A

Coupling:

Gi/Go

Protein Name:

GPR109A

Uniprot Number:

Q8TDS4

Protein Aliases:

niacin receptor 1|HCA2

Gene Name:

HCAR2

Gene ID:

338442

Gene Aliases:

HCA2|HM74A|PUMAG|Puma-g|NIACR1

Accession Number:

NM_177551

Organism:

Human

Cell Type:

CHO-K1

Assay Information

Assay Type:

Functional

Assay Sub Type:

Cell Based

Functional Mode:

Agonist

Testing Information

Control Activator:

Nicotinic Acid

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 10 concentrations are selected

Test Sample Requirements:

40uL of a 1000X stock for the first assay and 2uL for each additional assay (assay = 1 target, 1 readout, 1 mode)

Minimum Order Quantity:

20

Turnaround Time

Standard:

15 Business Days

Additional Information

Brand:

LeadHunter

Testing Location:

USA - Fremont, CA