Biological Information

Background Information:

cAMP Hunter™ Gi cell lines overexpress naturally Gi coupled, wild type GPCRs and are designed to detect inhibition of intracellular cAMP production in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay Detection Kit.

Family:

GPCR

Sub Family:

Class A Orphan

Class:

Orphan and other 7TM

Coupling:

Gi/Go

Protein Name:

GPR35

Uniprot Number:

Q9HC97

Protein Aliases:

KYNA receptor

Gene Name:

GPR35

Gene ID:

2859

Accession Number:

NM_005301

Organism:

Human

Cell Type:

CHO-K1

Assay Information

Assay Type:

Functional

Assay Sub Type:

Cell Based

Functional Mode:

Agonist

Testing Information

Control Activator:

Zaprinast

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 10 concentrations are selected

Test Sample Requirements:

40uL of a 1000X stock for the first assay and 2uL for each additional assay (assay = 1 target, 1 readout, 1 mode)

Minimum Order Quantity:

20

Turnaround Time

Standard:

15 Business Days

Additional Information

Brand:

LeadHunter

Testing Location:

USA - Fremont, CA