Biological Information
Background Information:
cAMP Hunter™ Gi cell lines overexpress naturally Gi coupled, wild type GPCRs and are designed to detect inhibition of intracellular cAMP production in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay Detection Kit.
Family:
GPCR
Sub Family:
Hydroxycarboxylic Acid
Class:
Class A
Coupling:
Gi/Go
Protein Name:
GPR81
Uniprot Number:
Q9BXC0
Protein Aliases:
G-protein coupled receptor 81|HCA1
Gene Name:
HCAR1
Gene ID:
27198
Gene Aliases:
GPR104|GPR81|HCA1|FKSG80
Accession Number:
NM_032554
Organism:
Human
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Agonist
Testing Information
Control Activator:
Sodium L-Lactate
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 10 concentrations are selected
Test Sample Requirements:
40uL of a 1000X stock for the first assay and 2uL for each additional assay (assay = 1 target, 1 readout, 1 mode)
Minimum Order Quantity:
20
Turnaround Time
Standard:
15 Business Days
Additional Information
Brand:
LeadHunter
Testing Location:
USA - Fremont, CA