H2 Human Histamine GPCR Cell Based Antagonist cAMP LeadHunter Assay - US

Background Information:

Histamine receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Histamine receptors are found in most peripheral tissue and within the central nervous system.

cAMP Hunter cell lines overexpress naturally coupled, wild type GPCRs and are designed to detect increases in intracellular cAMP levels (Gs-coupled receptors) or inhibition of intracellular cAMP production (Gi-coupled receptors) in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay Detection Kit. cAMP Hunter cell lines expressing receptors that will couple to Gq proteins can also be used to detect induction of calcium mobilization.

Family:

GPCR

Sub Family:

Histamine

Class:

Class A

Coupling:

Gq & Gs

Protein Name:

H2

Uniprot Number:

P25021

Protein Aliases:

HH2R|Gastric receptor I

Gene Name:

HRH2

Gene ID:

3274

Accession Number:

AY136744

Organism:

Human

Cell Type:

CHO-K1