Keap1-Nrf2 Human Transcription Factor Cell Based Agonist Nuclear Translocation LeadHunter Assay - US

Background Information:

PathHunter® Nuclear Translocation cell lines are engineered to co-express two proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA), which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-express an untagged secondary protein involved in the pathway of interest.

Family:

Pathways

Sub Family:

Transcription Factor

Class:

Basic Leucine Zipper Domain

Protein Name:

NRF2

Uniprot Number:

Q16236

Protein Aliases:

NFE2-related factor 2|HEBP1|Nuclear factor, erythroid derived 2, like 2

Gene Name:

NFE2L2

Gene ID:

4780

Gene Aliases:

NRF2

Accession Number:

NM_203500.1

Organism:

Human

Cell Type:

U2OS