M2 Human Acetylcholine (Muscarinic) GPCR Cell Based Antagonist Arrestin LeadHunter Assay - US

Background Information:

Muscarinic receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Muscarinic receptors are widely distributed in the central and peripheral nervous system as well as various tissues.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Acetylcholine (Muscarinic)

Class:

Class A

Coupling:

Gi/Go

Protein Name:

M2

Uniprot Number:

P08172

Protein Aliases:

acetylcholine receptor, muscarinic 2

Gene Name:

CHRM2

Gene ID:

1129

Accession Number:

AF498916

Organism:

Human

Cell Type:

CHO-K1