M4 Human Acetylcholine (Muscarinic) GPCR Cell Based Agonist cAMP LeadHunter Assay - US

Background Information:

Muscarinic acetylcholine receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Muscarinic acetylcholine receptors present in the central nervous system, spinal cord motoneurons and autonomic preganglia, modulate a variety of physiological functions. Five different subtypes of muscarinic receptors have been classified. Muscarinic M4 receptors are found in high levels in neuronal cells of the CNS, largely overlap those of M1 and M3 subtypes, and are preferentially located on striatal neurons that project to the internal globus pallidus influencing motor control.

cAMP Hunter™ Gs cell lines overexpress naturally Gs coupled, wild type GPCRs and are designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay Detection Kit.

Family:

GPCR

Sub Family:

Acetylcholine (Muscarinic)

Class:

Class A

Coupling:

Gs & Gi/Go

Protein Name:

M4

Uniprot Number:

P08173

Protein Aliases:

acetylcholine receptor, muscarinic 4

Gene Name:

CHRM4

Gene ID:

1132

Accession Number:

NM_000741.2

Organism:

Human

Cell Type:

CHO-K1