M4 Rat Acetylcholine (Muscarinic) GPCR Cell Based PAM Arrestin LeadHunter Assay - US

Background Information:

Muscarinic acetylcholine receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Muscarinic acetylcholine receptors present in the central nervous system, spinal cord motoneurons and autonomic preganglia, modulate a variety of physiological functions. Five different subtypes of muscarinic receptors have been classified. Muscarinic M4 receptors are found in high levels in neuronal cells of the CNS, largely overlap those of M1 and M3 subtypes, and are preferentially located on striatal neurons that project to the internal globus pallidus influencing motor control.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Acetylcholine (Muscarinic)

Class:

Class A

Protein Name:

M4

Uniprot Number:

P08485 

Protein Aliases:

acetylcholine receptor, muscarinic 4

Gene Name:

CHRM4

Gene ID:

25111

Accession Number:

NM_031547

Organism:

Rat

Cell Type:

CHO-K1