Motilin Human Motilin GPCR Cell Based Antagonist Arrestin LeadHunter Assay - US

Background Information:

Motilin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Muscarinic receptors are primarily distributed in the gastrointestinal tract and may also be present in the central nervous system.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization.

Family:

GPCR

Sub Family:

Motilin

Class:

Class A

Coupling:

Gq

Protein Name:

Motilin

Uniprot Number:

O43193

Gene Name:

MLNR

Gene ID:

2862

Gene Aliases:

GPR38|MTLR|MTLR1

Accession Number:

AY603964

Organism:

Human

Cell Type:

CHO-K1