OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
A colony formation or clonogenic assay has several key applications, including the assessment of irreversible cytotoxicity, reproductive toxicity, cancer/stem cell research, and therapeutic resistance.
This assay has the ability to measure cellular reproductive integrity, investigation of cancer-stemness, and is a widely used tool to predict the efficacy of particular anti-cancer therapeutics for cytotoxicity versus cytostasis. The assay will be performed in 96 well plates with 50 cells/well and treated with client compound at ten concentrations, in quadriplicates. Reference compound staurosporine or doxorubicin is used for comparison purpose in the assay. The colony is defined to consist of more than 50+ cells, which will be counted and reported as number of colonies and percent of control. Vehicle (DMSO or PBS) control will serve as percent of control values.
Organism:
Human
Construct Details:
Endogenous
Cell Type:
LS-174T
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Testing Information
Test Sample Requirements:
Minimum for Profiling: 200 µL of 1000X stock based on the top concentration to be tested per 50 cell lines tested -OR- Enough compound to make the described solution. Minimum 1mg.
Minimum Order Quantity:
40
Turnaround Time
Standard:
20 Business Days
Service Scheduling Note:
Turnaround time for 1 cell line is 20 business days. Turnaround time for > 7 cell lines is 30 business days.
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
OncoPanel®
Testing Location:
USA - St Charles, MO
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