OT Human Vasopressin / Oxytocin GPCR Cell Based PAM Arrestin LeadHunter Assay - US

Background Information:

The importance of oxytocin in parturition is based on its well-documented contractile effects on uterine myometrium at term; oxytocin receptors are also present in mammary gland especially during lactation. In addition to maintenance of labor, oxytocin is a key initiator of parturition at term and perhaps preterm. Parturition, however, is highly regulated and likely involves additional factors including estrogen, progesterone, prostaglandins, etc.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization.

Family:

GPCR

Sub Family:

Vasopressin and Oxytocin

Class:

Class A

Protein Name:

OT

Uniprot Number:

P30559

Gene Name:

OXTR

Gene ID:

5021

Accession Number:

AY389507

Organism:

Human

Cell Type:

CHO-K1