PAC1 (PACAP) Human VIP and PACAP GPCR Cell Based Antagonist cAMP LeadHunter Assay - US

Background Information:

Pituitary adenylate cyclase–activating polypeptide (PACAP) is a ubiquitous neuropeptide of the vasoactive intestinal peptide (VIP) family. PACAP exists in 2 forms; one as a 38 aa peptide (PACAP-38) and a C-terminally truncated, 27aa form of the peptide (PACAP-27). PACAP type I receptor has high affinity for both PACAP peptides and is expressed predominantly in the CNS (most abundantly in the olfactory bulb, thalamus, hypothalamus, the dentate gyrus of the hippocampus and in granule cells of the cerebellum.

Studies on PAC1 receptor knockout mice point to a role for presynaptic PAC1-mediated signaling at the mossy fiber synapse in long-term potentiation (LTP) and hippocampus-dependent associative learning. The PAC1 receptor is also expressed in brain areas which have been implicated in the emotional control of behavior, such as the amygdala, the hypothalamus, the locus coeruleus and the periaqueductal grey. Consistent with this, mice with a ubiquitous but not with a forebrain-specific deletion of the PAC1 receptor exhibited elevated locomotor activity and strongly reduced anxiety-like behavior. It is also expressed on pancreatic β-cells and has been implicated in the control of pancreatic insulin secretion.

cAMP Hunter cell lines overexpress naturally coupled, wild type GPCRs and are designed to detect increases in intracellular cAMP levels (Gs-coupled receptors) or inhibition of intracellular cAMP production (Gi-coupled receptors) in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay Detection Kit. cAMP Hunter cell lines expressing receptors that will couple to Gq proteins can also be used to detect induction of calcium mobilization.

Family:

GPCR

Sub Family:

VIP and PACAP

Class:

Class B

Coupling:

Gs & Gq

Protein Name:

PAC1 (PACAP)

Uniprot Number:

P41586

Protein Aliases:

PACAP-R-1|PACAP-R1|PACAP receptor 1

Gene Name:

ADCYAP1R1

Gene ID:

117

Gene Aliases:

PAC1|PACAPR|PAC1R

Accession Number:

AY366498

Organism:

Human

Cell Type:

CHO-K1