OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Cationic amphiphilic drugs (CADs), such as antibiotics, antidepressants, antihistamines and other prescription drugs, have been identified as inducers of phospholipidosis in animal and cell culture models. Drug-induced phospholipidosis is characterized by the appearance of intracellular phospholipids in lysosomes. Detection of drug-induced phospholipidosis has been performed using electron microscopy, a time-consuming and labor intensive technique, and/or quantitative PCR. Eurofins offers a rapid image-based high content analysis (HCA) assay to assess drug-induced phospholipidosis in HepG2 cells.
Organism:
Human
Construct Details:
Endogenous
Cell Type:
HepG2
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Detection Method:
Imaging / HCA
Platform:
MD ImageXpress® Micro
Measured Response:
Fluorescence
Testing Information
Incubation:
48 hr at 37C
Test Concentration / Dose:
Suggested to perform dose response study (8 concentrations; 3 replicates per concentration)
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
15 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
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