sst2 Human Somatostatin GPCR Cell Based PAM Arrestin LeadHunter Assay - US

Background Information:

Somatostatin (somatotropin-release inhibiting factor, SRIF) receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. The five known receptors can be divided into two groups: SRIF1 (SRIF1A=sst2, SRIF1B=sst5, srif1C=sst3) and SRIF2 (SRIF2A=sst1, srif2B=sst4); lower case (srif) indicates an as yet undefined physiological role. SRIF receptors are found in most peripheral tissues and within the central nervous system. Agonism at SRIF1A=sst2 receptors may inhibit release of growth hormone (GH), glucagon and acid secretion as well as tumor cell proliferation.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Somatostatin

Class:

Class A

Protein Name:

sst2

Uniprot Number:

P30874

Protein Aliases:

SS2-R|SS2R|SRIF-1

Gene Name:

SSTR2

Gene ID:

6752

Accession Number:

AY236542

Organism:

Human

Cell Type:

CHO-K1