OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction.
Family:
Kinase
Sub Family:
ACK
Class:
Protein Tyrosine
Protein Name:
TNK1
Uniprot Number:
Q13470
Protein Aliases:
CD38 negative kinase 1
Gene Name:
TNK1
Gene ID:
8711
Accession Number:
NM_003985.6
Organism:
Human
Construct Details:
106-390 recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Procedure Summary:
TNK1 (h) is incubated with 25 mM Tris pH 7.5, 0.2 mM EGTA, 0.5 mM sodium orthovanadate, 0.5 mM sodium βglycerophosphate, 0.01% Triton X-100, 1% Glycerol, 1 mM DTT, 0.33 mg/mL myelin basic protein, 10 mM Magnesium acetate and [γ-33P]-ATP (Specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 120 minutes at room temperature, the reaction is stopped by the addition of phosphoric acid solution to a concentration of 0.5%. An aliquot of the reaction is then spotted onto a filter and washed four times for 4 minutes in 0.425% phosphoric acid and once in ethanol prior to drying and scintillation counting.
Substrate:
0.33 mg/mL myelin basic protein
Tracer:
33P
ATP Concentration:
[ATP]= 90µM (Km= 89.2µM )
Incubation:
120 min at Room temperature
Control Inhibitor:
Staurosporine
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 50X stock based on the top concentration to be tested -OR- 100 μl of 10 mM stock -OR- 1.5 mg (pre-weighed). 2) Dose Response: 80 μl of 50X stock based on the top concentration to be tested -OR- 100 μl of 10 mM stock -OR- 1.5 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
6 Business Days
Service Scheduling Note:
Assay is run on a weekly schedule beginning on Friday. Compounds and purchase order must be received on or before 13:00 CET Thursday for the order to be included on the service run that begins on Friday of the same week.
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
KinaseProfiler
Testing Location:
KP - France - Celle L'Evescault
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