Tumor Cell Proliferation Assay in NCI-H460 Human Lung Cells, Panlabs

Procedure Summary:

NCI-H460 cells (1.5x10^4/ml in growth medium), human lung carcinoma (ATCC HTB-177), are placed in 96-well plates in an atmosphere of 5% CO2 at 37°C for overnight. Test compound and/or vehicle is then added for an additional 72 hours. After 72-hours incubation, luminescence is measured using a microplate reader. Growth inhibition (GI50), total growth inhibition (TGI) and lethal concentration of 50% (LC50) represent cell growth are 50% reduction of vehicle-treated control (T72), equal to and loss of cells of 50% relative to beginning cell density (T0) respectively.

Incubation:

3 d at 37°C

Control Inhibitor:

Staurosporine

Criteria For Significance:

≤ 50% Cell Growth

Test Concentration / Dose:

Compounds are screened at 30, 10, 3, 1, 0.3, 0.1, 0.03 and 0.01 μM

Test Sample Requirements:

75 μl of 10 mM stock or 1 mg (pre-weighed) powder (assuming FW ≦500 and purity 100%)

Minimum Order Quantity:

16