V1B Human Vasopressin / Oxytocin GPCR Cell Based Agonist Calcium Flux (in Arrestin Cell Line) LeadHunter Assay - US

Background Information:

Vasopressin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Vasopressin receptors are currently classified into V1A, V1B (V3) and V2 subtypes. The V1B receptor is known to be present only in the adenohypophysis. Vasopressin V1B receptor antagonism may inhibit ACTH release and be useful in the treatment of anxiety and depression.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization.

Family:

GPCR

Sub Family:

Vasopressin and Oxytocin

Class:

Class A

Coupling:

Gq

Protein Name:

V1B

Uniprot Number:

P47901

Protein Aliases:

AVPR V1b|AVPR V3|Antidiuretic hormone receptor 1b|Vasopressin V3 receptor

Gene Name:

AVPR1B

Gene ID:

553

Gene Aliases:

AVPR3|VPR3

Accession Number:

NM_000707.3

Organism:

Human

Cell Type:

CHO-K1