BioMAP Oncology Panel

Gain Actionable Insights with Tumor Microenvironment Models for Oncology and Immuno-Oncology

Eurofins Discovery experts developed our BioMAP® oncology/immuno-oncology systems to model the complexity of the tumor microenvironment (TME). The systems combine PBMCs pooled from healthy human donors with early passage primary human fibroblasts or vascular endothelial cells, together with a human tumor cell line. BioMAP oncology/immuno-oncology systems are stimulated to recapitulate and model tumor-immune-stromal or tumor-immune-vascular microenvironments with disease-relevant interactions and signaling pathways that occur during tumorigenesis and growth.
 
Discover how BioMAP oncology/immuno-oncology systems can help you gain insights into TME-specific efficacy, mechanism of action, dosing and indication guidance, and safety signatures of test agents in the complex TME environment.

Drug Discovery Applications

  • Biological mechanism of action, efficacy, and safety evaluation
  • Biomarker discovery
  • Benchmarking against standard of care, clinical drug, or reference agents
  • Testing of drug combinations for improving efficacy or reducing potential toxicity

BioMAP Oncology/Immuno-Oncology Panel Advantages: A Human-centered Approach

  • Use of human PBMCs, primary fibroblasts or vascular endothelial cells, and cancer cell line in co-culture to allow cell cross-talk
  • Tumor microenvironment markers relevant to human therapeutic response are studied: immune, vascular or stromal, and tumor
  • Additional custom protein biomarker readout options are also available

 

Oncology and Immuno-Oncology Systems
Service
  • Test agent (chemical compound or biologic) profiling in selected BioMAP systems
  • Four concentrations evaluated in biological triplicates
Description
  • Annotation Identification and biological interpretation of relevant BioMAP biomarker activities (readouts)
  • Benchmarking Direct comparison of test agent with a specified standard of care, clinical drug, or reference agent from the BioMAP database (list available upon request)
Deliverables
  • Study Report BioMAP profile plots, annotation of activities with respect to biological significance, graphical overlays of test agent and drug or reference agent profiles, expert data interpretation and analysis
  • Data Report Log ratio data tables, table of cytotoxicities, table of readouts

 

CRC Systems & Translational Biomarker Readouts for Immuno-Oncology
System Relevance Cell Types Translational Biomarker Readouts
StroHT29 Host Stromal-Tumor
Microenvironment,
Oncology, Oncology

CRC
HT-29 colorectal adenocarcinoma cell line +
Peripheral blood mononuclear cells +
Primary human fibroblasts
CD106/VCAM-1, CD87/uPAR, CEACAM5/CD66e, Collagen I, Collagen III, CXCL10/IP-
10, Keratin 20, MMP-9, PAI-I, PBMC Cytotoxicity, sGranzyme B, sIFN-γ, sIL-10, sIL-
17A, sIL-2, sIL-6, SRB, sTNFα, sVEGF, TIMP-2, tPA, uPA
VascHT29 Host Vascular-Tumor
Microenvironment,
Oncology, Oncology

CRC
HT-29 colorectal adenocarcinoma cell line +
Peripheral blood mononuclear cells +
Primary human endothelial cells
CCL2/MCP-1, CD106/VCAM-1, CD40, CD69, CD87/uPAR, CEACAM5/CD66e,
Collagen IV, CXCL10/IP-10, CXCL9/MIG, Keratin 20, PBMC Cytotoxicity, sGranzyme B,
sIFN-γ, sIL-10, sIL-17A

 
Eurofins Discovery Services and Eurofins DiscoverX Products provide a variety of targeted, phenotypic, and pathway-based solutions to enable you to make better targeted oncology or cancer immunotherapies, faster. Watch the webinar, Enabling Development of Cancer Immunotherapy Drugs.
 

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