Native Calcium Flux cell consists of overexpressed naturally Gq-coupled, wild type GPCR. The Calcium Flux assay can be used to monitor the activation of a GPCR via Gq second messenger in a live cell, non-imaging assay format.
Calcium mobilization in PathHunter cell lines expressing Gq-coupled GPCRs is monitored using a calcium sensitive dye that is loaded into cells. GPCR activation by a compound results in the release of calcium from intracellular stores and an increase in dye fluorescence that is measured in real time.
Calcium No WashPLUS Assay Principle
Calcium No WashPLUS Assay Principle. The esterified (inactive) calcium dye (probe) penetrates the cell membrane and becomes active once inside the cell. The active form of the dye becomes fluorescent after binding to intracellular calcium. Additive A is added to prevent the dye from being released out from the cell. Ligand binding stimulates GPCR activation, resulting in release of intracellular calcium stores from the ER, which leads to an increase in fluorescence in the presence of the activated calcium dye.
