Phosphatase Screening and Profiling In Vitro Assays

Eurofins Discovery has developed a series of phosphatase-specific enzymatic assays for your phosphatase-focused drug discovery programs. Our PhosphataseProfiler platform delivers a robust, convenient, reliable, and well-characterized solution to screen your focused chemical libraries and profile your lead compounds for their inhibitory effects on phosphatases.
 
Reversible protein phosphorylation is critical for normal cellular function with perturbations leading to diseases such as cancer. This post-translational modification alters protein function either by relocating proteins, changing protein interaction and/or modifying their enzymatic activity. Phosphatases are the key enzymes involved in removing phosphate groups and thus reversing the effect of kinase-directed phosphorylation.
 
As a therapeutic drug class, interest is growing in phosphatases such as PTP-1B as future therapeutic targets. As with kinases, phosphatase inhibitor specificity is a major challenge for drug discovery scientists. Many of our assays utilize DiFMUP as a universal substrate because this molecule has low fluorescence intensity in its non-hydrolyzed form, and removal of the phosphate group yields a highly fluorescent product, ensuring high-quality reproducible data.