sst4 Human Somatostatin GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR

Item: 2103

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OVERVIEW

DOCUMENTS/REFERENCES

Biological Information

Background Information:

Somatostatin (SS) is a neurotransmitter/hormone with a wide range of biological actions. Somatostatin mediates its diverse physiological effects through a family of five G-protein-coupled receptors (sst1-sst5). A reduction in cortical SS levels has been reported in Alzheimer’s and Parkinson’s diseases.

Family:

GPCR

Sub Family:

Somatostatin

Class:

Class A

Protein Name:

sst4

Uniprot Number:

P31391

Protein Aliases:

SS4-R|SS4R

Gene Name:

SSTR4

Gene ID:

6754

Accession Number:

NM_001052

Organism:

Human

Construct Details:

Recombinant

Cell Type:

CHO-K1

Assay Information

Assay Type:

Functional

Assay Sub Type:

Cell Based

Functional Mode:

Antagonist

Detection Method:

HTRF

Testing Information

Incubation:

10 min at 37°C

Control Activator:

somatostatin-14 (1 nM)

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

Turnaround Time

Standard:

15 Business Days

Clinical Relevance

Therapeutic Area:

Gastroenterology

Adverse / Beneficial Action:

A reduction in cortical SS levels has been reported in Alzheimers and Parkinsons diseases. The SS analog, octreotide (SMS 201995), is used clinically in the treatment of certain tumors, carcinoid syndrome, and glucagonoma. Somatostatin inhibits cell proliferation and growth hormone secretion. Somatostatin released from capsaicin-sensitive afferents exerts systemic anti-nociceptive actions, presumably via somatostatin receptor subtype 4 (sst4).

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault