OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Evaluation of the agonist activity of compounds at the human GPBA receptor expressed in transfected CHO cells, determined by measuring their effects on cAMP production using the HTRF detection method.
Family:
GPCR
Sub Family:
Bile Acid
Class:
Class A
Protein Name:
TGR5
Uniprot Number:
Q8TDU6
Protein Aliases:
hGPCR19|Membrane-type receptor for bile acids|M-BAR|hBG37|BG37
Gene Name:
GPBAR1
Gene ID:
151306
Gene Aliases:
BG37|GPCR|TGR5|M-BAR|GPCR19|GPR131|MGC40597
Accession Number:
NM_001077191
Organism:
Human
Construct Details:
Recombinant
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Agonist
Detection Method:
HTRF
Testing Information
Incubation:
30 min at RT
Control Activator:
Lithocholic Acid
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
GPBA (TGR5) Human Bile Acid GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
Item: 4093
GPBA (TGR5) Human Bile Acid GPCR Cell Based Agonist & Antagonist cAMP LeadHunter Assay - FR
Item: G242
SafetyScreen59 Functional Extended Panel - FR
Item: P458-EU
SafetyScreen33 Functional Delta Panel - FR
Item: P459-EU
SafetyScreen77 Functional Core Extended Panel - FR
Item: P460-EU
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