OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Evaluation of the antagonist activity of compounds at the human GPBA receptor expressed in transfected CHO cells, determined by measuring their effects on agonist-induced cAMP production using the HTRF detection method.
Family:
GPCR
Sub Family:
Bile Acid
Class:
Class A
Protein Name:
TGR5
Uniprot Number:
Q8TDU6
Protein Aliases:
hGPCR19|Membrane-type receptor for bile acids|M-BAR|hBG37|BG37
Gene Name:
GPBAR1
Gene ID:
151306
Gene Aliases:
BG37|GPCR|TGR5|M-BAR|GPCR19|GPR131|MGC40597
Accession Number:
NM_001077191
Organism:
Human
Construct Details:
Recombinant
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
HTRF
Testing Information
Incubation:
30 min at RT
Control Activator:
Lithocholic Acid (10 µM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
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SafetyScreen33 Functional Delta Panel - FR
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SafetyScreen77 Functional Core Extended Panel - FR
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