OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Muscarinic receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Muscarinic receptors are widely distributed in the central and peripheral nervous system as well as various tissues. A muscarinic M5 subtype antagonist may reduce dopamine D1 central nervous stimulation and inhibit salivary secretion.
Family:
GPCR
Sub Family:
Acetylcholine (Muscarinic)
Class:
Class A
Protein Name:
M5
Uniprot Number:
P08912
Protein Aliases:
acetylcholine receptor, muscarinic 5
Gene Name:
CHRM5
Gene ID:
1133
Accession Number:
NM_001320917
Organism:
Human
Construct Details:
Recombinant
Cell Type:
Chem-1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
Fluorimetry
Measured Response:
Fluorescence
Testing Information
Incubation:
RT
Control Inhibitor:
Atropine
Control Activator:
Acetylcholine (10 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 70 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Adverse / Beneficial Action:
A muscarinic M5 subtype antagonist may reduce dopamine D1 central nervous stimulation and inhibit salivary secretion.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
M5 Human Acetylcholine (Muscarinic) GPCR Binding Antagonist Radioligand LeadHunter Assay - FR
Item: 97
M5 Human Acetylcholine (Muscarinic) GPCR Cell Based Agonist Calcium Flux LeadHunter Assay - FR
Item: 4217
M5 Human Acetylcholine (Muscarinic) GPCR Cell Based Agonist & Antagonist Calcium Flux LeadHunter Assay - FR
Item: G033
Large Alzheimer Disease Discovery LeadHunter Panel - FR
Item: P426
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