Catechol-O-Methyltransferase Pig Enzymatic LeadHunter Assay - FR

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OVERVIEW

DOCUMENTS/REFERENCES

Biological Information

Background Information:

Most of the epinephrine and norepinephrine that enters the circulation, from the adrenal medulla or following administration, or that is released by exocytosis from adrenergic fibers, is methylated by catechol-o-methyltransferase (COMT) to metanephrine or normetanephrine, respectively. COMT (EC 2.1.2.6) is a soluble magnesium-requiring cytoplasmic enzyme that plays a major role in the metabolism of endogenous circulating and administered catecholamine.

Family:

Neurotransmitter Metabolism

Sub Family:

Catecholamine Turnover

Protein Name:

Catechol-O-Methyltransferase

Uniprot Number:

P21964

Gene Name:

COMT

Gene ID:

1312

Accession Number:

NM_000754

Organism:

Pig

Tissue:

Liver

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Fluorimetry

Measured Response:

Fluorescence

Testing Information

Substrate:

esculetin (1 µM)

Incubation:

15 min at 37°C

Control Inhibitor:

Ro 41-0960

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

21632

Name

5466-89-7

Measurement

2100nM - IC50

PubChem ID

3495594

Name

Ro 41-0960

Measurement

45nM - IC50

Clinical Relevance

Therapeutic Area:

CNS/Addiction/Pain

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault