Biological Information
Background Information:
Motilin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Muscarinic receptors are primarily distributed in the gastrointestinal tract and may also be present in the central nervous sytem.
Family:
GPCR
Sub Family:
Motilin
Class:
Class A
Protein Name:
Motilin
Uniprot Number:
O43193
Gene Name:
MLNR
Gene ID:
2862
Gene Aliases:
GPR38|MTLR|MTLR1
Accession Number:
NM_001507
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[125I]motilin
Ligand Kd (nM):
0.26
Ligand Concentration:
0.05 nM
Non Specific:
[Nleu13]-motilin (1 µM)
Incubation:
120 min at RT
Control Inhibitor:
[Nleu13]-motilin
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Gastroenterology
Adverse / Beneficial Action:
Motilin receptor agonism may accelerate gastric emptying and intestinal motility, which may be associated with nausea.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
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