Biological Information

Background Information:

Motilin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Muscarinic receptors are primarily distributed in the gastrointestinal tract and may also be present in the central nervous sytem.

Family:

GPCR

Sub Family:

Motilin

Class:

Class A

Protein Name:

Motilin

Uniprot Number:

O43193

Gene Name:

MLNR

Gene ID:

2862

Gene Aliases:

GPR38|MTLR|MTLR1

Accession Number:

NM_001507

Organism:

Human

Construct Details:

Recombinant

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Binding

Detection Method:

Radiometric

Measured Response:

Scintillation

Testing Information

Ligand:

[125I]motilin

Ligand Kd (nM):

0.26

Ligand Concentration:

0.05 nM

Non Specific:

[Nleu13]-motilin (1 µM)

Incubation:

120 min at RT

Control Inhibitor:

[Nleu13]-motilin

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

12560

Name

Erythromycin

Measurement

5500nM - IC50

PubChem ID

Name

[Nleu13]-motilin

Measurement

1.4nM - IC50

PubChem ID

Name

motilin

Measurement

0.63nM - IC50

Clinical Relevance

Therapeutic Area:

Gastroenterology

Adverse / Beneficial Action:

Motilin receptor agonism may accelerate gastric emptying and intestinal motility, which may be associated with nausea.

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

RELATED SOLUTIONS

Item: 1321
Item: 1894
Item: G084
Item: P22-p