V2 Human Vasopressin / Oxytocin GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Vasopressin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins, which constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Vasopressin receptors are currently classified into V1A, V1B (V3) and V2 subtypes. The V2 receptor is predominantly located in principal cells of the renal collecting duct system. Vasopressin
Family:
GPCR
Sub Family:
Vasopressin and Oxytocin
Class:
Class A
Protein Name:
V2
Uniprot Number:
P30518
Gene Name:
AVPR2
Gene ID:
554
Gene Aliases:
ADHR|DIR|DIR3|V2R
Accession Number:
XM_006724828
Organism:
Human
Construct Details:
Recombinant
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
HTRF
Testing Information
Incubation:
30 min at RT
Control Inhibitor:
ADAMANTANEACETYL-VASOPRESSING
Control Activator:
AVP (0.03 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Renal
Adverse / Beneficial Action:
V2 receptor antagonism may produce aquaresis (free water excretion without affecting electrolyte balance). Nephrogenic diabetes insipidus
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
V2 Human Vasopressin / Oxytocin GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item: 237
V2 Human Vasopressin / Oxytocin GPCR Binding Agonist Radioligand LeadHunter Assay - FR
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V2 Human Vasopressin / Oxytocin GPCR Cell Based Agonist & Antagonist cAMP LeadHunter Assay - FR
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