MC4 Human Melanocortin GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Melanocortins belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. The melanocortin MC1 receptor is made primarily in melanocytes, MC2 in adrenal cortex, MC3 and MC4 almost exclusively neuronal, and MC5 in exocrine gland cells.
Family:
GPCR
Sub Family:
Melanocortin
Class:
Class A
Protein Name:
MC4
Uniprot Number:
P32245
Gene Name:
MC4R
Gene ID:
4160
Accession Number:
NM_005912
Organism:
Human
Construct Details:
Recombinant
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
HTRF
Testing Information
Incubation:
30 min at 37°C
Control Inhibitor:
SHU 9119
Control Activator:
NDP-alpha -MSH (1 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Adverse / Beneficial Action:
Melanocortin MC4 receptor agonism may decrease body fat and attenuate cachexia while receptor antagonism may result in obesity and Type II diabetes.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
MC4 Human Melanocortin GPCR Binding Agonist Radioligand LeadHunter Assay - FR
Item: 420
MC4 Human Melanocortin GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item: 699
MC4 Human Melanocortin GPCR Cell Based Agonist & Antagonist cAMP LeadHunter Assay - FR
Item: G082
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