OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
T Cell specific stimulants (ConA, Anti-CD3 antibody, PHA or CD40L/IFNγ) are used to stimulate secretion of cytokines by human PBMCs and inhibition by client compound is reported as pg/mL for the strength of the response. Reference compound dexamethasone is used for comparison purpose. Non-treated cells serve as background control, while induced PBMCs in the presence of vehicle serve as a positive control. The number of PBMC donors in this study is customizable and can be tested as either individual or pooled donors. PBMCs from other species (NHP, rat, mouse, canine) with choice of stimulants are available upon client request.
Organism:
Human
Construct Details:
Endogenous
Cell Type:
PBMC
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
Luminex
Measured Response:
Fluorescence
Testing Information
Incubation:
24 or 48 hrs at
Control Inhibitor:
Dexamethasone
Control Activator:
ConA, Anti-CD3 antibody, PHA, or CD40L/IFNγ
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Turnaround Time
Standard:
20 Business Days
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
Panlabs
Testing Location:
USA - St Charles, MO
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