TLR1 /TLR2 Human TLR Cell Based Agonist Assay in PBMC

Item: 960031

Price: Contact For Pricing

Status: Available

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OVERVIEW

DOCUMENTS/REFERENCES

Biological Information

Background Information:

Client test articles are used on human PBMCs to monitor secretion of IL-6 and reported as pg/mL for the strength of the response. Pam3CSK4 (Diacylated Lipoprotein) is used to specifically activate TLR1/2 and is used as an induction control for comparison purposes, while non-treated cells serve as background control. The number of PBMC donors in this study is customizable and can be tested as either individual or pooled donors.

Family:

Cell Surface (Other)

Sub Family:

TLR

Class:

PRR

Organism:

Human

Construct Details:

Endogenous

Cell Type:

PBMC

Assay Information

Assay Type:

Functional

Assay Sub Type:

Cell Based

Functional Mode:

Agonist

Detection Method:

Luminex

Measured Response:

Fluorescence

Testing Information

Incubation:

24 hrs at

Control Activator:

Pam3CSK4

Test Sample Requirements:

Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).

Turnaround Time

Standard:

15 Business Days

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Adverse / Beneficial Action:

TLR ligands (agonists) have shown value for treatment and/or prevention of infectious and neoplastic diseases. Based on safety and efficacy of the TLR agonists used to date, applications are likely to increase in the future. TLR antagonists may be used to treat a number of inflammatory conditions, including sepsis, skin diseases, rheumatoid arthritis and systemic lupus erythematosus.

Additional Information

Brand:

Panlabs

Testing Location:

USA - St Charles, MO